Figure 2. H₂O₂ induces the expression of p21^Cip1

A: HLE B-3 cells were treated with 200 μM H₂O₂ at the indicated times. Cell lysates were prepared for western blot analysis. The expression of p21^Cip1, p27^Kip1, cdc2, cyclin A, cyclin D1, cyclin E, and actin were analyzed. B: Northern blot analysis for the induction of p21^Cip1 by 200 μM H₂O₂ at indicated times. C: HLE B-3 cells were treated for 16 h in either the absence or presence various concentrations of H₂O₂ as indicated. The cell lysates were prepared and analyzed in the level of p21^Cip1 by western blot. The data shown are representative of three independent experiments.