Figure 4. Elution profile of γ-crystallin using a S300 cation exchange column

Approximately 1 mg of γ-crystallin in 300 μl of Buffer A was resolved using a gradient of 100% Buffer A for 10 min, followed by a 0-30% linear gradient of increasing concentration of Buffer B for 15 min. Bold letters and numbers designate fractions collected and used for microequilibrium dialysis studies.