Figure 5 of
Fan, Mol Vis 2005;
11:76-87.Figure 5 of
Fan, Mol Vis 2005;
11:76-87.
Figure 5. Alignment of amino acid sequences of γ-crystallins
A: The amino acid sequences of rat γA-, γB-, γC-, γD-, γE-, γF-, and γS-crystallins were aligned. Greek key motifs I, II, III, and IV are indicated in brackets. Motif III a, b, c, and d β-strands are indicated in red. Asp 114 and Asn 138 positions of γE-crystallin are indicated. B: The amino acid sequences of mouse wild type γE-crystallin and the Elo mutant were aligned.
A:
I II
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gamma E MG----KITFYEDRGFQGRHYECSTDHSNLQPYFSRCNSVRVDSGCWMLYEQPNFTGCQYFLRRGDYPDYQQWMGFSDSVRSCRLIP-HS 85
gamma F ..----.............................................................................H...-.. 85
gamma D ..----.................................................................................-.A 85
gamma A ..----.............C....S.CP...T.......I...........R..YQ.Y.....................I....S..-YT 85
gamma B ..----....F........C....S.CP...T...................R..YQ.H.....................I.......Q.. 86
gamma C ..----.............C....S.CP...T.......I...........R..YQ.H.....................I.......-.T 85
gamma S .SKTGA..S.....N....R.D.DC.CVDFRS.L.....I..EG.T.AV..R...S.HM.I.PQ.E..E..R...LN.RLG...AVHLS. 90
114 138
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gamma E S-SHRIRIYEREDYRGQMVEITDDCPHLQDRFHFSDFHSFHVMEGYWVLYEMPNYRGRQYLLRPGEYRRYHDWGAMNARVGSLRRIMDFY 174
gamma F .-........................................I.....................R.......................Y. 174
gamma D G-.....L............F.E...S.......NEIY.LN.L..C......T................................V.... 174
gamma A .-.....L...D....LVS.L.E..SCIH...RLNEIY.M..L..S...................D..........D.K......V..L. 174
gamma B G-TY.M.....D.F....S......LS......L.EI..LN....C.......S................L....A..K...F..V.... 175
gamma C G-...M.L..K..HK.V.M.LSE..SCI.....L.EVR.L..L..C..................Q..........VD.KA.....VV.L. 174
gamma S GGQYK.Q.F.KG.FS...Y.T.E...STME...LREI..CK.L..T.IF..L.S.H......DKK...KPV....ASPA.Q.F...VE 178
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III IV
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B:
gamma E MGKITFYEDRGFQGRHYECSTDHSNLQPYFSRCNSVRVDSGCWMLYEQPNFTGCQYFLRRGDYPDYQQWMGFSDSVRSCRLIPHSSSHRI 90 gamma E ELO .......................................................................................... 90 gamma E RIYEREDYRGQMVEITDDCPHLQDRFHFSDFHSFHVMEGYWVLYEMPNYRGRQYLLRPGEYRRYHDWGAMNARVGSLRRIMDFY 174 gamma E ELO ............................................RCPTTGGGSTC 145 |