Figure 2 of Fan, Mol Vis 2005; 11:76-87.


Figure 2. Construction of expression vectors for MIP, tagged MIP, and tagged γ-crystallin proteins

A: Expression vector pCMV-MIP is used to express untagged MIP. B: pEGFP-MIP was constructed by fusing the full length MIP to the C-terminal end of EGFP. C: The full length rat γA-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γ-A crystallin. D: The full length rat γB-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γB-crystallin. E: The full length rat γC-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γC-crystallin. F: The full length rat γD-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γD-crystallin. G: The full length rat γE-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γE-crystallin. H: the full length rat γF-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γF-crystallin. I: The full length rat γS-crystallin was fused to the C-terminal end of HcRed to produce HcRed tagged γS-crystallin. J: The full length mouse γE-crystallin was linked to the C-terminal end of EGFP to produce EGFP tagged mouse γE-crystallin. K: the full length mouse γE-crystallin Elo mutant was fused to the C-terminal end of EGFP to produce EGFP tagged γE-crystallin Elo mutant. All expression vectors are under the control of a CMV promoter. MIP is indicated as a yellow box. γ-Crystallins are indicated as blue boxes. The EGFP tag is indicated as a green box. The HcRed tag is indicated as a red box. The Myc tag is indicated as a grey box.

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Fan, Mol Vis 2005; 11:76-87 <http://www.molvis.org/molvis/v11/a9/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535