Table 1 of
Delyfer, Mol Vis 2005;
11:688-696.Table 1 of
Delyfer, Mol Vis 2005;
11:688-696.
Table 1. RT-PCR conditions and primers
This table lists the oligonucleotides used for RT-PCR with the corresponding annealing temperatures, PCR product lengths, and GenBank accession numbers. All these primers were used for real-time PCR.
Annealing Product GenBank
Forward and reverse temperature length accession
Gene primer sequences (°C) (bp) number
-------- ---------------------------- ----------- ------- ---------
β-actin F: AAAGACCTCTATGCCAACACAG 57 296 M12481
R: AAAGAAAGGGTGTAAAACGCAG
GLAST F: GAAGTCTCCCAGACGTTCTAATCC 58 303 D63816
R: GCTCTGAAACCGCCACTTACTATC
GLT-1 F: ATGCTCATCCTCCCTCTTATCATC 56 313 AB007810
R: CTTTCTTTGTCACTGTCTGAATCTG
GS F: TGTACCTCCATCCTGTTGCC 56 349 U09114
R: GTCCCCGTAATCTTGACTCC
vimentin F: TTTTGCCCTTGAAGCTGCTAAC 56 314 M26251
R: TCACCTGTCCATCTCTGGTCT
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