Table 1 of Vasireddy, Mol Vis 2005; 11:665-676.


Table 1. Summary of ELOVL4 protein expression in COS-7 cells

Cells transfected with wild-type and/or mutant ELOVL4 constructs showed the presence of ELOVL4 fusion proteins of expected molecular mass. In presence of the mutant protein, the wild-type protein is misrouted to perinuclear aggresomes.

                                           Size of the
                         Localization   fragment observed
Constructs transfected   in the cells    on Immunoblots
----------------------   ------------   -----------------
GFP-Ewt                  ER               about 67 kDa
GFP-Emut                 Perinuclear      about 63 kDa
GFP-Ewt and GFP-Emut     Perinuclear      about 63 kDa
V5-Ewt                   ER               about 41 kDa
V5-Emut                  Perinuclear      about 37 kDa
GFP-Ewt and V5-Emut      Perinuclear      about 37 kDa
GFP-Emut and V5-Ewt      Perinuclear      about 37 kDa

Vasireddy, Mol Vis 2005; 11:665-676 <http://www.molvis.org/molvis/v11/a79/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535