Table 1 of
Vasireddy, Mol Vis 2005;
11:665-676.
Table 1. Summary of ELOVL4 protein expression in COS-7 cells
Cells transfected with wild-type and/or mutant ELOVL4 constructs showed the presence of ELOVL4 fusion proteins of expected molecular mass. In presence of the mutant protein, the wild-type protein is misrouted to perinuclear aggresomes.
Size of the Localization fragment observed Constructs transfected in the cells on Immunoblots ---------------------- ------------ ----------------- GFP-Ewt ER about 67 kDa GFP-Emut Perinuclear about 63 kDa GFP-Ewt and GFP-Emut Perinuclear about 63 kDa V5-Ewt ER about 41 kDa V5-Emut Perinuclear about 37 kDa GFP-Ewt and V5-Emut Perinuclear about 37 kDa GFP-Emut and V5-Ewt Perinuclear about 37 kDa |