Figure 1 of
Karan, Mol Vis 2005;
Figure 1. Nomenclature and diagrams of recombinant ELOVL4 constructs
A: EGFP and wild-type ELOVL4 fusion protein (wtEGFP/ELOVL4). B: EGFP fusion protein with the ER retention signal, KAKGD, replaced by AAAAA (mtEGFP/ELOVL4(5A)). C: DsRed2 and wild-type ELOVL4 fusion protein (wtDsRed/ELOVL4). D: DsRed2 and 5 bp deleted ELOVL4 mutant fusion protein (mtDsRed/ELOVL4(5bp-del)). E: DsRed2 and 270X ELOVL4 mutant fusion protein (mtDsRed/ELOVL4 (270X)) F: DsRed2 fusion protein with C-terminal ER retention signal, KAKGD, replaced with AAAAA (mtDsRed/ELOVL4(5A)). pCMV represents immediate early promoter from cytomegalovirus; EGFP represents enhanced green fluorescent protein; DsRed represents red fluorescent protein; pA represents polyadenylation signal from SV40 T-antigen; KAKGD/AAAAA represents dilysine motif replaced with alanine tract. All fluorescent proteins were fused in frame at the N-terminal end of the wild-type or mutant ELOVL4 amino acid sequence.