Figure 3 of
Gorbatyuk, Mol Vis 2005;
Figure 3. Immunostaining of retinas with vector expressing humanized GFP
Retinas of RHO+/- animals were injected at P6 with AAV2 GFP. Frozen sections were made along the vertical meridian and were reacted with anti-GFP polyclonal antibodies. Cells expressing GFP were detected using fluorescein labeled second antibody. The slide was counter stained with propidium iodide (red) to indicate the ONL and with antibody (B630) to rhodopsin visualized with Cy5-labled secondary antibody (blue). Transduced areas were measured from four such retinas and ranged from 60-70% of the full retina, with the central retina predominantly transduced.