Figure 2 of
Gorbatyuk, Mol Vis 2005;
Figure 2. Rz397 leads to opsin mRNA degradation in vitro
HEK 293 cells were transfected with a plasmid expressing canine rhodopsin mRNA using a CMV immediate early promoter. Cells were co-transfected with a plasmid expressing an irrelevant ribozyme only (white bar) or with a plasmid expressing Rz397 using the CMV-chicken β-actin (CBA) hybrid promoter at a molar ratio of 6:1 (red bar) or 8:1 (blue bar). It should be noted that the CMV promoter is more potent than the CBA promoter in these cells (S. Zolotukhin, unpublished results). Levels of RHO transcript were measured in triplicate using reverse transcription PCR in the presence of α-[32P]-dATP and were normalized to levels of β-actin mRNA amplified in the same reactions. Products were analyzed by gel electrophoresis and phosphorescence scanning. The p values for RHO:Rz 1:6 and RHO:Rz 1:8 are <0.00038 and <0.0046, respectively.