Figure 1. Kinetic analysis of ribozyme Rz397

A: Hammerhead ribozyme 397 was designed to cleave following residue 397 in dog rhodopsin mRNA and in the analogous position (333) in mouse rhodopsin mRNA. B: Using an oligonucleotide substrate in tenfold excess of ribozyme, Rz397 reached a cleavage plateau at approximately 10 min in 5 mM MgCl₂. The data points are from duplicate determinations that varied by less than 10%. C: Using 10 nM ribozyme, k_obs was measured as a function of substrate concentration (from 0.1 to 15 μM). The data points are the average of duplicate determinations that varied by less than 10%. The V_max was determined to be 19.5 nM/min and the K_M was 910 nM using a double reciprocal plot of these data (not shown).