Table 1 of Scheef, Mol Vis 2005; 11:613-624.


Table 1. Screening primers

These primers were used for mouse genotyping by PCR. The PCR conditions were as follows: 94 °C 3 min, and 35 cycle of 94 °C 20 s, 59 °C 10 s, and 72 °C 45 s. The PCR products were analyzed on a 2% agarose gel. The TSP1 reverse primer was used with the Neo forward primer for the mutant allele or TSP1 forward primer for the wild-type allele. The wild-type and mutant alleles yield PCR products of 700 and 400 bp, respectively. The Neo and immorto primer pairs yield PCR products of 650 and 350 bp, respectively.

 Name                   Primer
-------   -----------------------------------
immorto   F: 5'-CCTCTGAGCTATTCCAGAAGTAGTG-3'
          R: 5'-TTAGAGCTTTAAATCTCTGTAGGTAG-3'

Neo       F: 5'-TGCTCTCCATCTGCACGAGACTAG-3'
          R: 5'GAGTTGCTTGTGGTGAACGCTCAG-3'

TSP1      F: 5'-AGGGCTATCTGGAATTAATATCGG-3'
          R: 5'-GAGTTTGCTTGTGGTGAACGCTCAG-3'

Scheef, Mol Vis 2005; 11:613-624 <http://www.molvis.org/molvis/v11/a73/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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