Figure 4. Expression of other cell adhesion molecules in retinal astrocyte cultures

Expression of CD47 (A,B), ICAM-1 (C,D), VCAM-1 (E,F), and SHPS-1 (G,H) was determined using FACS analysis as described in Methods. The shaded graphs show staining in the absence of primary antibody (control IgG). Note decreased expression of CD47, ICAM-1, and VCAM-1 in TSP1^-/- RAC, while expression of SHPS-1 was similar in these cells. Each of these experiments was performed at least three times with similar results.