Table 1 of Zhao, Mol Vis 2005; 11:603-608.


Table 1. Human specific primer sequences and sizes of real-time RT-PCR

The table lists the sequences (5' to 3') of the forward and reverse primers used to amplify the various splice variant forms of versican from V0 to V3. The size of the amplicon that was made by the real-time RT-PCR for each isoform is given as base pairs (bp).The designation "F" indicates forward and "R" indicates reverse.

                                           Amplicon
Gene           Sequence (5'-3')            size (bp)
----   ---------------------------------   ---------
 V0    F: GCACAAAATTTCACCCTGACATT             836
       R: CTTCTTTAGATTCTGAATCTATTGGATGAC

 V1    F: CCCAGTGTGGAGGTGGTCTAC               124
       R: CGCTCAAATCACTCATTCGACGTT

 V2    F: TCAGAGAAAATAAGACAGGACCTGATC         135
       R: CATACGTAGGAAGTTTCAGTAGGATAACA

 V3    F: CCCTCCCCCTGATAGCAGAT                 71
       R: GGCACGGGGTTCATTTTGC

Zhao, Mol Vis 2005; 11:603-608 <http://www.molvis.org/molvis/v11/a71/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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