Table 1 of
Zhao, Mol Vis 2005;
11:603-608.
Table 1. Human specific primer sequences and sizes of real-time RT-PCR
The table lists the sequences (5' to 3') of the forward and reverse primers used to amplify the various splice variant forms of versican from V0 to V3. The size of the amplicon that was made by the real-time RT-PCR for each isoform is given as base pairs (bp).The designation "F" indicates forward and "R" indicates reverse.
Amplicon Gene Sequence (5'-3') size (bp) ---- --------------------------------- --------- V0 F: GCACAAAATTTCACCCTGACATT 836 R: CTTCTTTAGATTCTGAATCTATTGGATGAC V1 F: CCCAGTGTGGAGGTGGTCTAC 124 R: CGCTCAAATCACTCATTCGACGTT V2 F: TCAGAGAAAATAAGACAGGACCTGATC 135 R: CATACGTAGGAAGTTTCAGTAGGATAACA V3 F: CCCTCCCCCTGATAGCAGAT 71 R: GGCACGGGGTTCATTTTGC |