Figure 3. Recovery of reduced ubiquinone

Mixtures (10 μg total) of reduced and oxidized Ub-9 (A) and reduced and oxidized Ub-10 (B) were subjected to direct HPLC analysis (Before Extraction) and HPLC analysis after being carried though the homogenization and extraction procedure (After Extraction) used to recover lens ubiquinones. The ratio of reduced to oxidized was compared before and after extraction. Vertical arrows mark the solvent front. Horizontal arrows mark one minute. Tracings are from right to left.