Table 1 of
Brinkmann, Mol Vis 2005;
11:582-586.Table 1 of
Brinkmann, Mol Vis 2005;
11:582-586.
Table 1. Experimental parameters used in this study
PCR primers and predicted optimal temperatures for dHPLC analysis of the corresponding amplicons.
PCR primers Optimal
Gene Fragment (forward/reverse) temp (°C)
----- -------- --------------------- -----------
VAMP5 Exon 2 CACTCCGCACACATCATACC 61.3
TCTAGGCTATGGGTGCCTGA
Exon 3, GACAAGATGGGGAGGATGC 62.1
part 1 GCTCTGAGGGAGAAAGACGA
Exon 3, GCTCATCATCCTGATTGTGCT 59.4
part 2 GTGGAGTCCTTTGGGTGATG
VAMP8 Exon 2 CTCACCTTCTGGGGCTTACA 60.4
GTCAAACTTCTGGCCCTCCT
Exon 3, TGTGGGCTGCACTTGTACTC 59.6
part 1 CCGAGCAGCATTCTCTGTAG
Exon 3, CAGGGACAACCCTCCATAAA 57.6
part 2 TCTTTGCCCTTCCAACAACT
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