Table 1 of
Jablonski, Mol Vis 2005;
11:569-581.Table 1 of
Jablonski, Mol Vis 2005;
11:569-581.
Table 1. Primers for PCR amplification of the candidate gene Rs1h
Listed in the table are the sequences for the primers used to amplify the mouse Rs1h gene from genomic or cDNA, the DNA melting temperature (Tm), and the predicted fragment size of the PCR product. cDNA was used as a template with primers pairs Rs1h-3L/Rs1h-4R and Rs1h-L/Rs1h-R. Genomic DNA was used as a template with primer pairs Rs1h-5UTR-L/Rs1h-Intron1-R and Rs1h1iL/Rs1h2iR.
Fragment
Designation Sequence (5'-3') Tm (°C) size (bp)
-------------- ------------------------------- ------- ---------
Rs1h-3L TGGCTATGAAGCCACATTGGG 63 °C 679
Rs1h-4R CCCAAAGCTCTCCCTGCAAGTG
Rs1h-L CACTTAGATCTTGCTGTGACCAAGGAC 65 °C 190
Rs1h-R CAGACCACAGAGCATTGGCTCC
Rs1h-5UTR-L CTTAATCTCTATGGCATTGTTTTCATTTTGC 66 °C 326
Rs1h-Intron1-R CTCATGCCCACACCCAACACC
Rs1h1iL TGCCCTGCTCCTATGCCAGC 46 °C 244
Rs1h2iR TACCCCTCAGCACTCTTCCCC
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