Figure 5. Sequencing analysis of Rs1h

The amplified cDNA of a wild-type mouse and two amplification products of different sizes from a 44TNJ mutant mouse were sequenced. A: Wild-type control. B: Sequence of transcript 1 from the 44TNJ mutant of normal size, showing a double sequence caused by a splice variant with a 10 bp deletion. The beginning of the double sequence is marked with an arrow. A third sequence with smaller peaks starting at position 63 corresponds to transcript 2. C: Sequence of transcript 2 from the 44TNJ mutant with a 26 bp deletion.