Figure 4. Chaperone activity of αL-crystallin against heat-induced aggregation of CS or DTT induced aggregation of insulin

Chaperone activity of αL-crystallin as assessed by the suppression of heat induced aggregation of CS (A) or DTT induced aggregation of insulin (B). CS (0.05 mg/ml) or insulin (0.40 mg/ml) was incubated in the absence (trace 1) or presence of αL-crystallin from Group I (trace 2), Group II (trace 3), Group III (trace 4), and Group IV (trace 5). The CS and insulin assays used 0.025 and 0.50 mg/ml αL-crystallin, respectively. Data were the average of three chaperone assays.