Figure 3. Chaperone activity of α-crystallin against heat-induced aggregation of βL-crystallin

Chaperone activity of αL- (A) and αH-crystallin (B) as assessed by the suppression of heat-induced aggregation of βL-crystallin. βL-Crystallin (0.2 mg/ml in 50 mM phosphate buffer, pH 7.4) was incubated at 65 °C in the absence (trace 1) or in the presence of either αL- or αH-crystallin (0.025 mg/ml) from Group I (trace 2), Group II (trace 3), Group III (trace 4), and Group IV (trace 5). Data were the average of three chaperone assays.