Figure 2 of
Kumar, Mol Vis 2005;
Figure 2. Separation profile of αH- and αL-crystallin on gel filtration
Protein (80 mg) was loaded onto a Sephacryl S-300 HR column. The column was equilibrated with TNEN buffer and proteins were eluted with the same buffer at 0.2 ml/min flow rate. Fractions were monitored at 280 nm.