Table 1 of
van der Spuy, Mol Vis 2005;
11:542-553.Table 1 of
van der Spuy, Mol Vis 2005;
11:542-553.
Table 1. Retinal markers used on control and LCA retina
Retinal markers used on control and LCA retina are listed. Antibodies used in immunohistochemistry of paraffin embedded sections included Ab-hAIPL1, A233, 1D4, COS-1, OS-2, LUMIf, and Recoverin. The sections were also labeled with the lectins PNA and WGA. The staining patterns for each marker in the control and LCA retinas are described.
Predominant staining pattern
-------------------------------------------------
Retinal marker Control adult retina LCA adult retina
----------------------- ------------------------ ----------------------
Ab-hAIPL1 [16,17] Rod photoreceptors No signal
excluding rod
outer segments
A233 (Glial fibrillary Astrocyte processes at Reactive Müller cell
acidic protein) the ILM processes
1D4 (Rhodopsin) [17] Rod photoreceptor outer No signal
segments
COS-1 (Long/medium L/M-cone outer segments No signal
wavelength-sensitive
(L/M)-cone opsin) [16]
OS-2 (Short S-cone outer segments No signal
wavelength-sensitive
(S)-cone opsin) [16]
PNA (Peanut agglutinin) Cone photoreceptor outer Photoreceptor residual
[17] and inner segment ECM inner segment ECM
sheaths sheaths
WGA (Wheatgerm Cone and rod Photoreceptor residual
agglutinin) photoreceptor outer inner segment ECM
and inner segment ECM sheaths
sheaths
LUMIf (Cone arrestin) Cone photoreceptors Photoreceptor residual
[22,23] inner segments
Recoverin (N-16; Rod Rod and cone Photoreceptor residual
and cone recoverin) photoreceptors; weak inner segments and INL
bipolar cell staining
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