Figure 4. Labeling of the control and LCA retina with the lectins WGA and PNA

Double immunofluorescent confocal microscopy of the control and LCA retina labeled with WGA (red) and PNA (green). The retinal nuclei were counterstained with DAPI (blue). The control (A) and LCA (E) retinal sections were visualized with bright field and differential interference contrast (Nomarski) optics, and the immunofluorescent confocal Z sections and bright field images were overlaid (A,E). The control (B-D) and LCA (F-H) sections were also visualized at higher magnification without Nomarski optics. The control and LCA sections were labeled with WGA shown in red (B,F), PNA shown in green (C,G), and colocalization of the WGA and PNA label shown in yellow (asterisks) were visualized by merging the images of the confocal Z sections (D,H). The photoreceptor outer segments (OS), photoreceptor inner segments (IS), outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) are labeled. The scale bars represents 20 μm.