Table 1 of
Jin, Mol Vis 2005;
11:535-541.Table 1 of
Jin, Mol Vis 2005;
11:535-541.
Table 1. Primer sets
Shown are the primers designed to amplify the corresponding fragments US-1, US-2, US-3, A, C, D, and Del. A minus sign was used to designate nucleotides upstream of the reference ATG, in which the "A" of the ATG is taken as position +1. The "Del" primer pair amplified the segment spanning the suspected deletion region, and; the forward primer was located upstream of the deletion while the reverse primer was downstream.
Product Location
Primer Tm length ---------------------------------
name Sequence (°C) (bp) 5' end (bp) 3' end (bp)
------ ------------------------ ---- ------- --------------- ---------------
US-1 F: TGGTGTTCCTGGAAAGAGTCA 60 1106 Upstream, -1227 Upstream, -122
R: CGAAACTCCGGAGATCAACT
US-2 F: GCCTCCCGACCCTATTCTAC 60 1036 Upstream, -2101 Upstream, -1066
R: AAGCGTTGACCTGGTGTAGG
US-3 F: AGGGTTCCTGTGAAGATTTGA 60 1113 Upstream, -3102 Upstream, -1990
R: GGAGGCACGTATTTGAGGAA
A F: AGCGGCTGTTTGTTGACTTC 56 211 Upstream, -175 Exon 1, +36
R: CAACTCACCGGGCATCAG
C F: CCTTGCATTCCCAAGCTC 56 152 Upstream, -209 Upstream, -57
R: GTCGGGGCTAAAGCAGCTA
D F: GGCGCGTGGTCTCTACTG 56 206 Upstream, -302 Upstream, -96
R: CGAAAGCCCCCAAGTTCC
Del F: ATGGGAAACCCTGCATCATA 54 890 Upstream, -799 IVS11, +193
R: ATGCTGCTGTGGTTACATGG
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