Figure 5 of
Tasheva, Mol Vis 2005;
11:452-460.
Figure 5. Subcellular localization of B7 and mimecan proteins
A,B: MG-63 cells transfected with pEGFP.B7 construct and examined by confocal microscopy. B7 was found to localize to both nucleus and cytoplasm. C: MG-63 cell were transfected with pcDNA-Exp40/Bovine mimecan, incubated with primary mouse anti-V5 antibody followed by incubation with FITC conjugated secondary antibody and examined by confocal microscopy. Mimecan localizes to the cytoplasm of MG-63 cells. D: MG-63 cell were transfected with pcDNA-Exp40/Bovine mimecan, incubated with primary mouse anti-V5 antibody followed by incubation with Alexa Fluor 546 conjugated secondary antibody and examined by confocal microscopy. Mimecan localizes to cytoplasm of MG-63 cells.