Table 2 of
Ishikawa, Mol Vis 2005;
11:431-437.Table 2 of
Ishikawa, Mol Vis 2005;
11:431-437.
Table 2. Sequences of primary probes and Invader oligonucleotides
Nine polymorphisms were detected among all participants. These polymorphisms are listed at GeneCanvas. Genotyping of the polymorphisms was performed by the Invader® assay using the probes listed below.
Base
Polymorphism Location changes Target Probe Sequence
------------- -------- ------- ------ ------- --------------------------------------------
EDN1/T-1370G 5' T/G Anti- T Flap1-TTGGTGGAGAACAAACAA
flanking sense G Flap2-GTGGTGGAGAACAAACA
region Invader GGTCTTACTGGGCCACTGTGAGCGCTC
EDN1/+138/ex1 Exon 1 del/ins Sense A del Flap1-TAACGGGGAGAAAAGG
del/ins A ins Flap2-TTAACGGGGAGAAAAGG
Invader GCGATCCTTCAGCCCAAGTGCCCTTC
EDN1/G8002A Intron 4 G/A Anti- G Flap1-GAAAATCATTTTGGGGAGC
sense A Flap2-AAAAATCATTTTGGGGAGC
Invader TGCCTCTCTGAGTCAATGTATTTACCACTTTCCCTGAGAAATCT
EDN1/K198N Exon 5 G/T Sense G Flap1-CTTGCCTTTCAGCTTGG
T Flap2-ATTGCCTTTCAGCTTGG
Invader GTTGTGGGTCACATAACGCTCTCTGGAGGGT
EDNRA/G-231A Exon 1 G/A Sense G Flap1-CTCCTGGGCACTGC
A Flap2-TTCCTGGGCACTGC
Invader CTGCACAGCTTCCCCGGCTTCAGAAAACA
EDNRA/H323H Exon 6 T/C Anti- T Flap1-TTTAAGCCGTATATTGAAGAAAA
sense C Flap2-CTTAAGCCGTATATTGAAGAAAA
Invader CTTGGTTGTAATTTTTGCTCTTTGCTGGTTCCCTCTTCAA
EDNRA/C+70G Exon 8 C/G Sense C Flap1-GTCACAGTTGCCTTGT
G Flap2-CTCACAGTTGCCTTGT
Invader GGAAGAAGGATCAGAGAAGAGATTCCCGGAT
EDNRA/C+1222T Exon 8 C/T Anti- C Flap1-CTTGGGGTTTTCAGTATGA
sense T Flap2-TTTGGGGTTTTCAGTATGA
Invader CCCACAAATGCCACCAGAACTTAACGATTCTTCACTTA
EDNRB/L277L Exon 4 A/G Anti- A Flap1-ATTCAGTTTCTATTTCTGCTTG
sense G Flap2-GTTCAGTTTCTATTTCTGCTTG
Invader CTCATCCCTATAGTTTTACAAGACAGCAAAAGATTGGTGGCTT
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