Figure 2. Labeling of glutathione peroxidase 3 mRNA by in situ hybridization

A: RPE/choroid sections from a 2-month-old C57BL/6 mouse labeled with antisense RNA. B: RPE/choroid sections from a 24-month-old C57BL/6 mouse labeled with antisense RNA. C: RPE/choroid sections from a 24-month-old C57BL/6 mouse labeled with sense RNA. The digoxigenin labeled in situ hybridization reaction with BCIP/NBT appears blue-purple. Arrows show glutathione peroxidase 3 (GPX3) labeled RPE cells. GPX3 labeled choroidal cells (arrowhead) were seen in sections of the 2-month-old mouse (A) and the 24-month-old mouse (B) Only a few representative positive cells are marked with arrows or arrowheads. No labeled cells were observed in the RPE or choroid with a sense RNA probe (C). The scale bar represents 50 μm.