Table 1 of
Salvador-Silva, Mol Vis 2005;
11:356-365.Table 1 of
Salvador-Silva, Mol Vis 2005;
11:356-365.
Table 1. Oligonucleotide primers
Primer sets for the listed genes were selected, as indicated in Methods, from their corresponding published cDNAs (GenBank accession number). The predicted length for each of the PCR DNA products in base pairs (bp), annealing temperature, and location of the forward and reverse sequence for each primer pair are also listed.
Product Annealing GenBank
length temperature accession
Gene Primers (bp) (°C) number Location
---------- ------------------------------ ------- ----------- --------- ---------
ABCR 5'-GTGCTCCCGGCTACCTTTGTGTTT-3' 824 60 NM_000350 4132-4155
5'-TACTCCTCGGGGCTCCTGTCCTTA-3' 4932-4955
11-cis-RDH 5'-ACCCTTGCCCTGCTGCCTCTGC-3' 415 63 U43559 430- 451
5'-TGTAGCGGGTTCGGGGGTGTCG-3' 823- 844
CRBP 5'-CGCAAAATCGCCAACTT-3' 288 56 M11433 216- 232
5'-GACCACACCTTCCACTCTCAT-3' 485- 503
LRAT 5'-GCCCCGGACCCACCTGACC-3' 456 61 AF071510 159- 177
5'-GACGCCAATCCCAAGACTGCTGAA-3' 591- 614
IRBP 5'-CCCCGAGCCTCCCCCACAAG-3' 505 62 M22453 400- 419
5'-GGCCCCTCCCCCAGTCC-3' 904- 888
CRALBP 5'-TGGCGGAGAGGGTGCAAGAGAAGG-3' 458 65 L34219 251- 274
5'-TTTGAACCGGGCTGGGAAGGAATC-3' 708- 685
RPE65 5'-GTCACGCTCCCCAATACAACT-3' 400 55 AH006489 1150-1170
5'-CAGCCCGGGCAACTTCACT-3' 1549-1531
β-actin 5'-TCGTGCGTGACATTAAGGAGAAGC-3' 506 60 BC013835 695- 718
5'-CTAGAAGCATTTGCGGTGGACGAT-3' 1200-1177
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