Figure 5. Reporter activity of CRALBP and IRBP promoter constructs in bovine PE and NPE cells

A: CRALBP-luciferase (Luc) reporter constructs (p2.1-Luc [-2089 to +80 bp] and p0.2-Luc [-200 to +80 bp]). B: IRBP chloramphenicol acetyl transferase (CAT) reporter constructs (p156 [-156 to +101], p70 [-70 to +101] and p45 [-45 to +101]). The transcriptional activity generated by the CRALBP-Luc and IRBP-CAT constructs were normalized to β-galactosidase and expressed as relative light units (RLU) and CAT activity (fold pvoCAT), respectively. Basic (pGL2) and pvoCAT indicate two control vectors used in the transfection assay lacking promoter regions for CRALBP and IRBP genes, respectively. Bars represent the mean and standard error of the mean for at least three independent experiments. Asterisks indicate statistical significance calculated using Student's t-test (p<0.05)