Figure 4. Expression of RPE65 mRNA and protein in the ciliary epithelium

A: The expected DNA product sizes amplified by PCR for RPE65 (400 bp) and β-actin (506 bp) were amplified from cDNA templates, synthesized in vitro from poly (A)+ mRNA prepared from bovine RPE (lane 1) and ciliary process (lane 2). DNA products were resolved on 1% agarose gels, stained with ethidium bromide, and their identity confirmed by direct sequencing. B: A representative western blot analysis of the microsomal fractions from bovine RPE (lane 1) and ciliary process (lane 2), with polyclonal anti-peptide antibody to RPE65. A β-actin antibody was used to verify loading of protein in each lane. Arrow in B indicates the migration of RPE65.