Figure 1 of
Salvador-Silva, Mol Vis 2005;
11:356-365.
Figure 1. Detection of components of the retinoid cycle in human ciliary processes and in a human ciliary epithelial cell line
Sets of oligonucleotide primers were selected to amplify by RT-PCR coding regions from the following genes; ATP binding cassette, retina (ABCR), 11-cis-retinol dehydrogenase (RDH), cellular retinol binding protein (CRBP), lecithin:retinol acyltranferase (LRAT), interphotoreceptor retinoid binding protein (IRBP), and cellular retinaldehyde binding protein (CRALBP). DNA products amplified by PCR were resolved on 1.2% agarose gels, stained with ethidium bromide, and photographed. Lane 1 represents retina, lane 2 represents ciliary processes, and lane 3 represents the ODM-C4 cell line. To the left of each gel is a 1 Kb DNA ladder marker (M).