Figure 6 of
Liu, Mol Vis 2005;
11:321-327.
Figure 6. Trp fluorescence of βB2-crystallin
The Trp fluorescence of βB2-crystallin provides information about the environment of the Trp residue. The red-shift of the Trp emission wavelength indicates exposure of Trp residues due to partial unfolding of βB2-crystallin. The relative fluorescence of untagged WT βB2-crystallin, 6xHis tagged WT βB2-crystallin, and the 6xHis tagged Q155* mutant is shown. The excitation wavelength is λex=295 nm. Protein concentrations are 0.08 mg/ml.