Table 1 of
Hawse, Mol Vis 2005;
11:274-283.Table 1 of
Hawse, Mol Vis 2005;
11:274-283.
Table 1. Primers used for RT-PCR
The table lists the sequences, GenBank accession numbers, annealing temperatures, product lengths, and abbreviations for all gene specific primers used in this study.
Annealing Product
temperature length Accession
Gene (Abbreviation) Primer sequence (5'-3') (°C) (bp) number
----------------------------- ------------------------ ----------- ------- ---------
Aquaporin 1 (AQP1) AGCCTCTTATGGGGGTGTTTCTAT 56 417 NM_000385
ATTCCAAGGTCAGGCAGTAAGTCC 56 417 NM_000385
Chloride channel (CLCA2) AGCCTGGGCACTGGACTTACA 56 388 NM_006536
GGGGTGCTTATGCTGGGAGAG 56 388 NM_006536
EST GTGAAGGCATCCCTGGTAGA 60 321 BF513244
TGGCTGAGCTGGTCCTTAAT 60 321 BF513244
AAGCCACATATGCCTCCAAG 60 336 AI189753
CATGAAAACTGTGGCAAACG 60 336 AI189753
Gap junction protein alpha 1 AGGCGGGAAGCACCATCTCTA 56 372 NM_000165
(GJA1) CCACCCATCTACCCCCATACACC 56 372 NM_000165
Glutathione synthetase (GSS) ATTTGACCAGCGTGCCATAGAGA 56 372 NM_000178
AGTGAGTAGAGGCCAGCAAAGGTG 56 372 NM_000178
Hypothetical protein CGTAGCCTCATTCCCAGGTA 60 375 AL136727
TGTTTGCTCCTGAGGCTTTT 60 375 AL136727
GGCCGTATCAAGGAGATTCA 60 349 NM_018104
CCCTTTGACGTAGGAAACCA 60 349 NM_018104
TTGATGTCAACATGGGCTGT 60 372 NM_017803
TGGCTTGTCGAAAGTCCTCT 60 372 NM_017803
Insulin-like growth factor TCTGCTCACCCAGAGACCTT 60 368 AW007532
binding protein 5 (IGFBP5) GAAGGGGCATGATCAGAAAA 60 368 AW007532
Laminin receptor 1 (LR1) CGCTCCAGTCTTCAGTAGGG 60 383 AW304232
CTACCCTACCATTGCGCTGT 60 383 AW304232
Lens epithelial protein TCCTCCTCTGCTGCTGGTGTATCA 61 426 NM_018655
(LENEP) GGTTTCTTAGGGGGCAGTCCATTC 61 426 NM_018655
Metallothionein Ig (MTIg) GCCTCTTCCCTTCTCGCTTG 56 217 NM_005950
GACATCAGGCGCAGCAGCTG 56 217 NM_005950
Metallothionein Ih (MTIh) GAACTCCAGTCTCACCTCGG 56 213 NM_005951
GACATCAGGCACAGCAGCTG 56 213 NM_005951
Nuclear receptor coactivator TCAATGCAGGTGAACAAAGTG 60 342 AI040324
2 (NRC2) TTCCTACATAGCTGTCCGATG 60 342 AI040324
PAX 6 (PAX6) CAGGGCAATCGGTGGTAGTAAA 56 520 NM_000280
TTCTCGGGCAAACACATCTGGA 56 520 NM_000280
Peroxiredoxin 6 (PRDX6) TCCAACCATCCCTGAAGAAGAA 56 386 NM_004905
AGCCCTGCAGAGATCCAACAAG 56 386 NM_004905
Protocadherin gamma TTGGTTTGGATCTCGGTAGC 60 368 NM_002588
subfamily c (PCDHGC3) TCGGCTCAGCTCATAGGTTT 60 368 NM_002588
Tissue inhibitor of GGGCTGTGCAACTTCGTGGAGA 61 333 NM_000362
metalloproteinase 3 (TIMP3) CAGCGGGAAGGGAGGGAAGTGA 61 333 NM_000362
Transcription elongation GCGGTTGAAGTGTAGGGAGA 60 340 X57198
factor A (TFIIS) AGTCTTGGCCATCTGATGCT 60 340 X57198
Unknown protein GGTGACTTCACACGCCATAA 60 302 BC001224
TCGAGTTGTCCACAGTCAGC 60 302 BC001224
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