Table 2 of
van den Hurk, Mol Vis 2005;
11:263-273.
Table 2. Primers used to determine the expression profile for CRB1, CRB2, and GAPDH.
Expression profiles for CRB1, CRB2, and GAPDH were determined by RT-PCR on RNA samples of eleven human tissues and RPE cell line ARPE-19. To rule out genomic contamination, forward and reverse primers for each gene were designed in different exons.
Gene Exon Sequence (5'>3')
----- ---- ------------------------
CRB1 10 TGCAGACAGAGCAGATTACC
11 AGCAAAGGCAACATCACAGAGG
CRB2 12 TCTGCAATGCCAGATTCTCCGG
13 CGGTGGCACCTTGAGGACACTGTC
GAPDH 8 ACCACAGTCCATGCCATCAC
9 TCCACCACCCTGTTGCTGTA
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