Table 2 of
van den Hurk, Mol Vis 2005;
11:263-273.
Table 2. Primers used to determine the expression profile for CRB1, CRB2, and GAPDH.
Expression profiles for CRB1, CRB2, and GAPDH were determined by RT-PCR on RNA samples of eleven human tissues and RPE cell line ARPE-19. To rule out genomic contamination, forward and reverse primers for each gene were designed in different exons.
Gene Exon Sequence (5'>3') ----- ---- ------------------------ CRB1 10 TGCAGACAGAGCAGATTACC 11 AGCAAAGGCAACATCACAGAGG CRB2 12 TCTGCAATGCCAGATTCTCCGG 13 CGGTGGCACCTTGAGGACACTGTC GAPDH 8 ACCACAGTCCATGCCATCAC 9 TCCACCACCCTGTTGCTGTA |