Table 1 of
van den Hurk, Mol Vis 2005;
11:263-273.Table 1 of
van den Hurk, Mol Vis 2005;
11:263-273.
Table 1. PCR primers for verification of the CRB2 gene prediction
Four overlapping nested primer sets were designed from the CRB2 gene prediction and used for RT-PCR on human retina cDNA. Primary PCR products were diluted and used as a template for the nested PCR reactions.
Fragment Exon Forward primer (5'>3') Reverse primer (5'>3') --------- ----- ------------------------------- -------------------------------- 1 primary 1> 7 CTCCCGTTCTCACAGCAGCCGA CACAGGACCAGAGGCCACACAGAGC 1 nested 1> 7 GGGCTGCCATGGCGCTGGCC AGCTCCAAGCTTTCCTTGGTGTCATTGCGAG 2 primary 7> 8 ACTGAGGTTTCGCACCACACTGCC CCACCCCAGGGCTGGCTGTC 2 nested 7> 8 GGAAAGCTTGGAGCTGGCATTGGTGGCAGC CGGATCCGACCCTCACTCAGGAA 3 primary 7>10 AACGTCCTCCTGGGCTGTGAGC ACGCGAAGTGCTCTCGGGCG 3 nested 8>10 TCCGCTGGCCTGTTGCTCCA AGCAGGATGCGCACAGCACCCGGGCCCTGCAG 4 primary 10>13 CTGGCACCGCGTGCGTCTGG GAAACCATTCAGGACCTCCAGGTGC 4 nested 10>13 TGTGCGCATCCTGCTGGCTGAGAACTTCACC CCTAGATGAGTCTCTCCTCCGG |