Figure 5 of
van den Hurk, Mol Vis 2005;
11:263-273.Figure 5 of
van den Hurk, Mol Vis 2005;
11:263-273.
Figure 5. Alignment of non-cbEGF-like domains
Alignment of the non-cbEGF-like domains of Drosophila (Dm) Crumbs (CRB; M33753), human (Hs) CRB1 (AY043325), and human (Hs) CRB2 (AY720432). Amino acid substitutions identified in the non-cbEGF-like domains of CRB2 in RP and LCA patients are indicated under the alignment. Identical amino acids are indicated in red and conserved residues in blue. Components of each line: organism, protein name, number of EGF-like domain, N-terminal amino acid position of the EGF-like domain, amino acid sequence of the EGF-like domain.
Dm CRB EGF1 267 -----NFC---LND-PCMGHGTCS-----SSPE--GYECRCTARYSGKNCQKDN
Dm CRB EGF2 305 ----GSPC---AKN-PCENGGSCLE----NSRG--DYQCFCDPNHSGQHCETEV
Dm CRB EGF3 345 --NIHPLC---QTN-PCLNNGACV-----VIGGSGALTCECPKGYAGARCEV--
Dm CRB EGF6 464 -----MTC---QTQ-QCLNGGTCL-----DKPI--GFQCLCPPEYTGELCQIA-
Dm CRB EGF7 501 -----PSC-----AQQCPIDSECVGGKCVCKPGSSGYNCQTSTGDGASALALTP
Dm CRB EGF8 545 -----INCN--ATNGKCLNGGTCS------MNG---THCYCAVGYSGDRCEKA-
Dm CRB EGF9 582 -----ENCS--PLN--CQEPMVCV-----------QNQCLCPE---NKVCNQ--
Dm CRB EGF10 611 -------C---ATQ-PCQNGGECVD----LPNG--DYECKCTRGWTGRTCGN--
Dm CRB EGF19 979 ----LKGC---DQN-PCLNGGACLPYLINEVTH--LYNCTCENGFQGDKCEKT-
Dm CRB EGF20 1207 -----EQC---KPN-PCHSNGECT-----DLWH--TFACHCPRPFFGHTCQHN-
Dm CRB EGF21 1480 ----DDLC---RKN-PCLHNAECR-----NTWN--DYTCKCPNGYKGKNCQ---
Dm CRB EGF22 1516 ---EIEFC----QHVTCPGQSLCQN---LD-DG---YECVTN------------
Dm CRB EGF23 1759 ------LC---FQS-DCKNDGFCQ-----SPSD--EYACTCQPGFEGDDCGT--
Dm CRB EGF26 1872 ----QMTC---ENE-PCRNGSTCQNGFNASTGN--NFTCTCVPGFEGPLCD---
Dm CRB EGF27 1913 ----IPFC---EIT-PCDNGGLCL-----TTGA--VPMCKCSLGYTGRLCEQ--
Dm CRB EGF30 2030 -----DPCN--ATD-LCSNGGRCVE----SCGAKPDYYCECPEGFAGKNCTAP-
Hs CRB1 EGF1 30 ---NNTRC---LSN-SCQNNSTCK----DFSKD-NDCSCSDTANNLDKDCDN--
Hs CRB1 EGF2 70 ---MKDPC---FSN-PCQGSATCV-----NTPGERSFLCKCPPGYSGTICET--
Hs CRB1 EGF3 110 ---TIGSC---GKN-SCQHGGICH-------QDPIYPVCICPAGYAGRFCEI--
Hs CRB1 EGF8 301 ---LMPLC---WSK-PCHNNATCE-----DSVD--NYTCHCWPGYTGAQCEI--
Hs CRB1 EGF11 441 ---ILLGC---THQ-QCLNNGTCIPHFQ-DGQH--GFSCLCPSGYTGSLCEI--
Hs CRB1 EGF12 672 ---RKDWC---ESQ-PCQSRGRCI-----NLWL--SYQCDCHRPYEGPNCLR--
Hs CRB1 EGF13 887 ---GDNSC---KSN-PCHNGGVCH-----SRWD--DFSCSCPALTSGKACEE--
Hs CRB1 EGF14 925 ----VQWC---GFS-PCPHGAQCQP----VLQG---FECIANAVFNG-------
Hs CRB1 EGF15 1139 ---QLNVC---NSN-PCLHGGNCE-----DIYS--SYHCSCPLGWSGKHCEL--
Hs CRB1 EGF18 1252 SRLPSTVCGNEKTNLTCYNGGNCT-----EFQT--ELKCMCRPGFTGEWCEK--
Hs CRB2 EGF1 38 ---PPSAC---ASD-PCAPGTECQA----TESG--GYTCGPM------------
Hs CRB2 EGF2 67 ---EPRGC---ATQ-PCHHGALCVP----QGPDPTGFRCYCVPGFQGPRCEL--
Hs CRB2 EGF10 396 ---QLTGC---QGH-TCPLAATCIP---IFESGVHSYVCHCPPGTHGPFCGQ--
Hs CRB2 EGF11 605 ---RREQC---RPL-PCVHGGSCV-----DLWT--HFRCDCARPHRGPTCAD--
Hs CRB2 EGF12 807 ---SEDMC---SPD-PCFNGGTC---L--VTWN--DFHCTCPANFTGPTCAQ--
Hs CRB2 EGF13 845 ----QLWC---PGQ-PCLPPATCE----EVPDG---FVCVAEATFRE-------
Hs CRB2 EGF14 1056 ---GAPVC---APS-PCLHDGACR-----DLFD--AFACACGPGWEGPRCEA--
Hs CRB2 EGF15 1094 ---HVDPC---HSA-PCAR-GRCH-----THPD-GRFECRCPPGFGGPRCRL--
Hs CRB2 EGF16 1134 ---PSKECS--LNV-TCLDGSPCE------GGS-PAANCSCLEGLAGQRCQV--
Hs CRB2 EGF17 1173 ---PTLPC---EAN-PCLNGGTCR-----AAGG--VSECICNARFSGQFCEV--
| | | |
| P46L T1110M V97L
R610W
|