Figure 4. Effect of Arg·HCl on the denaturation of bovine α-crystallin by urea at 25 °C

The open circle indicates α-crystallin alone, the closed circle indicates α-crystallin plus 300 mM Arg·HCl. The α-crystallin concentration was 0.2 mg/ml, the excitation wavelength was 295 nm, and the excitation and emission band passes were each set at 5 nm.