Figure 4. Immunolocalization of CNTFRα in the adult pig retina

A: Negative control (pigmented RPE). B,C: Pattern of immunoenzymatic labeling with the anti-chick CNTFRα antibody at 1:2,000 (B) or 1: 10,000 (C) dilutions. D: Sequential section labeled with human cone arrestin antibody. E,F: Double immunofluorescence labeling (overlaid images) with the anti-chick CNTFRα (red) and COS1 (green, E) or OS2 (green, F) antibodies. Intense immunoenzymatic labeling with the CNTFRα antibody (1:2,000 dilution) was seen at the inner segments (IS), outer nuclear layer (ONL; cone cell bodies), outer plexiform layer (OPL), inner nuclear layer (INL), ganglion cell layer (GCL), and nerve fiber layer (NFL; B). Labeling with the CNTFRα antibody at a 1:10,000 dilution was still present in the cone cell bodies (arrows, C), and resembled that obtained with the human cone arrestin antibody (arrows, D). CNTFRα immunolabeling of M/L (E) and S (F) cones was localized to their inner segments, cell bodies (arrows), axons (arrowheads), and pedicles (open arrowheads). Scale bars represent 20 μm.