Table 1. Overall cell survival after 120 h in culture

All surviving cells (Calcein AM positive; TOTO3 negative) with and without neurites were counted. Analysis of five experiments demonstrates no significant differences in overall cell survival at 120 h, normalized to growth media alone although a trend towards increased survival was noted with BDNF, BMP2, and GDF8. To account for multiple comparisons, p<0.007 was considered significant. Using the number of live cells at the time of plating as the initial live number, the average survival with no additional growth factors was 44.0%±6.6%. There were 59 wells used for each sample.

                                   Comparison
                                    to media
            Survival   Standard       only
Treatment    ratio     deviation   (p value)
---------   --------   ---------   ----------
  Media       1.00        0.21
  BDNF        1.10        0.23      0.01323
  BMP2        1.08        0.20      0.03913
  BMP13       0.99        0.26      0.84960
  GDF8        1.11        0.21      0.00738
  TGFA        0.88        0.37      0.03222
  TGFB1       0.99        0.26      0.89964
  TGFB2       0.99        0.32      0.87170