Figure 1. CC, CXC, and CX3C chemokine receptor gene expression in giant papillae in atopic keratoconjunctivitis

A shows an autoradiograph of CC chemokine receptors and B shows an autoradiograph of CXC/CX3C chemokine receptors. Specific bands for each chemokine were identified from undigested probe migration patterns. Lane M represents an undigested marker probe. Lane N is normal conjunctivae. Lanes 1-5 correspond to the five patients. C,D: Normalized analysis results are shown of the autoradiographs from A and B. The vertical axes have arbitrary units based on densitometery. The bands were normalized to the liposome 32 (L32) housekeeping gene. In comparison to chemokine receptor mRNA levels in normal conjunctivae, CCR4 mRNA is abundant in four out of five AKC patients (C). In CXC and CX3C chemokine receptor, CXCR4 mRNA levels are high in the giant papillae of all five patients (D). Two sets of results on separate experiments were analyzed, and averaged density of the individual bands was shown as the results of densitometric analysis.