Table 1 of
Choudhary, Mol Vis 2005;
Table 1. Source and concentration of antibodies used in protein analysis
TSP1 and TSP2 protein expression was determined in sham infected and HSV1 infected human keratocyte cultures by immunohistochemical staining and western blotting. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH), an enzyme involved in glycolysis, is widely expressed by all living cells. Its expression by mock infected and HSV1 infected keratocytes was used as a control for western blot analysis. HSV1 infected cell protein (HSV1 1CP27) was used to determine the expression of immediate early viral proteins and HSV1 glycoprotein D (HSV1gD) was used for the detection of late viral proteins. The HRP conjugated antibodies were the secondary antibodies used in the immunohistochemical procedures.
Antibody Source Dilution --------------- ---------- -------- TSP1 (N-20) sc-12312 1:100 Santa Cruz TSP2 (N-20) sc-7655 1:100 Santa Cruz GAPDH-6C5 AB-8245 1:2000 ABcam HSV1 ICP27 sc-17544 1:100 (vp-20) Santa Cruz HSV1gD sc-17540 1:100 (vn-20) Santa Cruz Anti-goat IgG A4416 1:1000 (HRP conjugate) Sigma Anti-mouse IgG A5420 1:2000 (HRP conjugate) Sigma