Table 1. Source and concentration of antibodies used in protein analysis

TSP1 and TSP2 protein expression was determined in sham infected and HSV1 infected human keratocyte cultures by immunohistochemical staining and western blotting. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH), an enzyme involved in glycolysis, is widely expressed by all living cells. Its expression by mock infected and HSV1 infected keratocytes was used as a control for western blot analysis. HSV1 infected cell protein (HSV1 1CP27) was used to determine the expression of immediate early viral proteins and HSV1 glycoprotein D (HSV1gD) was used for the detection of late viral proteins. The HRP conjugated antibodies were the secondary antibodies used in the immunohistochemical procedures.

   Antibody         Source     Dilution
---------------   ----------   --------
TSP1 (N-20)       sc-12312      1:100
                  Santa Cruz

TSP2 (N-20)       sc-7655       1:100
                  Santa Cruz

GAPDH-6C5         AB-8245       1:2000
                  ABcam

HSV1 ICP27        sc-17544      1:100
(vp-20)           Santa Cruz

HSV1gD            sc-17540      1:100
(vn-20)           Santa Cruz

Anti-goat IgG     A4416         1:1000
(HRP conjugate)   Sigma

Anti-mouse IgG    A5420         1:2000
(HRP conjugate)   Sigma