Table 1 of
Jiang, Mol Vis 2005;
11:143-151.Table 1 of
Jiang, Mol Vis 2005;
11:143-151.
Table 1. Oligonucleotide primers used for amplification and sequencing of exons 1-4 of the human GUCA1A (GCAP1) gene
Primers marked with "F" are forward (sense) primers, while those marked with "R" are reverse (antisense) primers. Amplicons generated with F/R pairs were directly sequenced to identify mutations in the GCAP1 gene.
Exon Primer sequence -------- ------------------------------- GCAP1_1F 5'-GGCCTGTCCATCTCAGACGT-3' GCAP1_1R 5'-CCCCAGCTGGTCAGGCTTCCAG-3' GCAP1_2F 5'-GCCTGAGGCTGGAGTGAGCG-3' GCAP1_2R 5'-CTAACCCTGGGCTCTCAGTTCC-3' GCAP1_3F 5'-CCTGAGATAGGATAAGGATGG-3' GCAP1_3R 5'-ACCCCACATCCATGGTGACC-3' GCAP1_4F 5'-CTGGACTGCAGAAATGAACACCCTC-3' GCAP1_4R 5'-GGCGAGCTAAGCCTCTGAGTTC-3' |