Figure 3 of
Jiang, Mol Vis 2005;
11:143-151.
Figure 3. The EF4 Ca2+ binding site and sequence alignment of vertebrate GCAPs
A: The Ca2+ binding loop consisting of 12 amino acids is flanked by two a-helical regions. Residues in the loop that coordinate the Ca2+ ion are marked with an asterisk. Although the 151 position amino acid (arrow) does not directly coordinate the Ca2+ ion, it is highly conserved throughout the GCAPs (leucine or isoleucine) suggesting it is essential for structure and function of this loop. B: The known vertebrate guanylate cyclase activating proteins (vertebrate GCAP1-8) have been aligned (only the C-terminal halves containing EF3 and EF 4 are shown). Residues conserved in more than 50% of the sequences shown are red. Conservative substitutions are blue (for origin of sequences and accession numbers, see Methods).
B:
L151F I143NT | E155G Y99C | | | | EF3 | EF4 | | |------------ |------------ hGCAP1 94 WYFKLYDVDGNGCIDRDELLTIIQAIRAINPCSDTTMTAE---------EFTDTVFSKIDVNGDGELSLEEFIEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGADEAAEAAG bGCAP1 94 WYFKLYDVDGNGCIIRDELLTIIRAIRAINPCSDSTMTAE---------EFTDTVFSKIDVNGDGELSLEEFMEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGASGRETEAAEADG mGCAP1 94 WYFKLYDVDGNGCIDRDELLTIIRAIRTINPWSDSSMSAE---------EFTDTVFAKIDINGDGELSLEEFMEGVQKDQMLLDTLTRSLDLTGIVRRLQNGEHEEAGTSDLAAEAAG ratGCAP1 94 WYFKLYDVDGNGCIDRDELLTIIRAIRTINPWSDSSMSAE---------EFTDTVFAKIDINGDGELSLEEFMEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQEEAGAGDLAAEAAG fGCAP1 93 WYFKLYDVDGNGCIDRGELLNIIKAIRAINRCN-DEMTAE---------EFTDMVFDKIDINGDGELSLEEFIEGVQKDELLLEVLTRSLDLKHIVYMIQNDGKRMEISERPRQEITTGNSLP cGCAP1 93 WYFKLYDVDGNGCIDRGELLNIIKAIRAINRCN-EAMTAE---------EFTNMVFDKIDINGDGELSLEEFMEGVQKDEVLLDILTRSLDLTHIVKLIQNDGKNPHAPEEAEEAAQ oryGCAP1 93 WYFKLYDVDGNGCIDRHELLNIIKAIRAINGNENQEMTAE---------EFTNNVFDRIDVNGDGELSLEEFVEGARSDEDFMEVMMKSLDLRHIVAMIHNRRHSV fuguGCAP1 93 WYFKLYDVDGNGCIDRHELLNIIKAIRAINGNENHETTAE---------DFTNSVFDRIDINGDGELSLEEFVAGARSDDVFMEVMIKSLDLTHIVAMIHNRRHSV zGCAP1 93 WYFRLYDVDGNGCIDRYELLNIIKAIRAINGSETQESSAE---------EFTNRVFERIDINGDGELSLDEFVAGARSDEEFMEAMMKSLDLTHIVAMIHNRRHSV bGCAP2 99 WTFKIYDKDRNGCIDRQELLDIVESIYKLKKACSVEVEAEQQGKLLTPEEVVDRIFLLVDENGDGQLSLNEFVEGARRDKWVMKMLQ--MDLNPSSWISQQRRKSAMF hGCAP2 96 WTFKIYDKDGNGCIDRLELLNIVEGIYQLKKACRRELQTEQD-QLLTPEEVVDRIFLLVDENGDGQLSLNEFVEGARRDKWVMKMLQ--MDMNPSSWLAQQRRKSAMF mGCAP2 96 WTFKIYDKDRNGCIDRLELLDIVEAIYKLKKACRAELDLEHQGQLLTPEEVVDRIFLLVDENGDGQLSLTEFIEGARRDKWVMKMLQ--MDINPGGWITQQRRRSAMF fuguGCAP2 95 WTFKMYDKDGSGCIDKTELLEIVESIYRLKKACHGELDEDCT--LLTPDQVVDRIFELVDENGDGELSLDEFIDGARRDKWVMKMLQ--MDVNPGDWLND-RRRSADF oryGCAP2 95 WTFKMYDKDGSGCIDKTELLEIVESIYRLKKACHGELDEECN--LLTPDQVVDRIFELVDENGDGELSLDEFIDGARRDKWVMKMLQ--MDVNPGDWINE-RRCSEDF zGCAP2 94 WTFKMYDKDGSGCIDKTELKEIVESIYRLKKACHGELDAECN--LLTPDQVVARIFELVDENGDGELSLDEFIAGARRDKWVMKMLQ--MDVNPGDWINEQRRRSANF fGCAP2 94 WTFKVYDRDGNGCIDKTELLEIVESIYNLKKVCRQGQDDRIP--LLSPEQVVDRIFQLVDENGDGQLSLDEFIDGARKDKWVMKMLQ--MDVSPGSWINEQRRKSALF cGCAP2 95 WTFKVYDKDGNGCIDKPELLEIVESIYKLKKVCRSEVEERTP--LLTPEEVVDRIFQLVDENGDGQLSLDEFIDGARKDKWVMKMLQ--MDVNPGGWISEQRRKSALF siluGCAP2 92 WSFKIYDKDGNGCVDKRELKEIIQSIYSIKRGWRR--DQEAQ--LMSPEEICERIFQIVDENGDGQLSLQEFVEGAXKDTWVLKMLQ--LDTNPCNWVMEQRRKSALF fuguGCAP6 93 WSFKVYDRDNNGFVDRTELRSIIDSIHRIKK------DSGSQ---LTVDEVVDRIFQAADSDGDGYISAEEFIRGAQQDPWLLNILK--LDMNPAGWVMEQRRKSAHF fuguGCAP7 92 WSFKMYDKDGNGKLDRQEVKRLIKIIHKIKLQRSDINMTP--------SEICDRIFELVDNNNDGQISLSEFMEGAQKDEWVMNLLK--LDVNATSWVLHNCEKLP fuguGCAP8 94 WSFKVYDRDGNGCLDKQEVRHIVKIIHKIK-KKSDMSVTEKI------EDVCDRIFELVDKNKDSQISLEEFIEGAEKDPWLMNQLR--LDIGPCEWFIEQREKKL fuguGCAP5 92 WYFKLYDIDGSGCIDRDELLLIIKSIRAINGIP-SEMSAE---------EFTNMVFDKIDINGDGELSYEEFIEGIQNDETLLKMLTESLDLTHIMQKIEGQMNASSSD zGCAP5 93 WYFKLFDMDGSGCIDKDELLLIFKAVQAINGAE-PEISAE---------DLADMVFNKIDVNGDGELSLEEFMEGISADEKISEMLTQSLDLTRIVSNIYNDSYIEQEAEIIEDQA zGCAP3 92 WYFKLFDQDGNGKIDRDEMETIFKAIQDITRSY--EIPPD---------DIVSLIYERIDVNNEGELTLEEFITGAKEHPDIMEMLTKMMDLTHVLEIIVNGQKKKKE fuguGCAP3 92 WYFKLFDQDGNGKIDKDELETIFKAIQDITRTY--DIPPE---------EIVTLIYDKIDVNGEGELTLEEFISGAKEHPDIMEMLTKMMDLSHVLEIIIKGQKKNTVK zGCAP4 92 WYFKLFDQDGNGKIDKDELETIFTAIQDITRNR--DIVPE---------EIVALIFEKIDVNGEGELTLEEFIEGAKEHPEIMDMLKILMDLTPVLLIIVEGRQK fuguGCAP4 92 WYFKLFDQDGNGKIDREELETIFSAIQDITRNR--DIDPE---------EIVSLIFERIDVNGEGELTLEEFIEGAKDHPDIMDTLKKIMDLTPVLVIIVEGRTG hGCAP3 95 WYFKLYDADGNGSIDKNELLDMFMAVQALNGQQ--TLSPE---------EFINLVFHKIDINNDGELTLEEFINGMAKDQDLLEIVYKSFDFSNVLRVICNGKQPDMETDSSKSPDKAGLGKVKMK fGCIP 94 WSFKLYDKDGDGAITRSEMLEIMRAVYKMSVVASLTKVNPMT-----AEECTNRIFVRLDKDQNAIISLQEFVDGSLGDEWVRQMLE--CDLSTVEIQKMTKHSHLPARSSRERLFHANT fuguGCIP 95 WSFKLYDKDRDGGITRQEMLEIMQAVYKMSLAAALTRPNPLT-----AEECTNRVFARLDRDNNAIISLEEFIEGALDDDWIREMLE--CDPTTVKVERPLRRDAVLGIHG ------------ ------------ EF3 EF4 |