Table 3 of
Kraft, Mol Vis 2005;
**11**:1246-1256.

Table 3. Single photon events provide estimates of wild type and mutant rhodopsin content in the outer segment

An estimation of the proportion of wild type and mutant rhodopsin
molecules present in the outer segment can be constructed by sorting of
dim flash responses (n=96) into blanks (no response; n=36), step-like
photocurrent responses (n=29), single-photon responses (n=21), and
combination responses (n=10). First, we assume mutant rhodopsin produces
the step-like responses, wild type rhodopsin produces "singles", and a
Poisson distribution of events. Then one can vary the probability of
mutant rhodopsin (p_{Ser347}) and wild type rhodopsin (p_{wt}) in
order to produce the observed distribution. The predictions below are
based on p_{Ser347} equal to 0.58 and p_{wt} equal 0.42. The
number of observed blank responses permits calculating μ, the mean
number of events per flash using the Poisson equation.

Calculated μ=0.98 -------------------- Predicted Observed --------- -------- Step-like currents 28.6 29 Singles 17.3 21 Combinations 13.6 10 |

Kraft, Mol Vis 2005;

©2005 Molecular Vision <http://www.molvis.org/molvis/>

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