Table 1 of Rex, Mol Vis 2005; 11:1236-1245.


Table 1. Concentrations of enhanced green fluorescent protein in retinal cells

Column 1 specifies the means by which the enhanced green fluorescent protein cDNA was delivered. Genomic delivery was accomplished through microinjection of zygote pronuclei; column 2 lists the promoter; column 3 gives the cell type. Column 4 gives the estimated EGFP concentration (Figure 2); the values are mean±SEM, with the average taken over the number of cells indicated in column 5. Column 6 shows the numbers of retinas analyzed. The final column provides ratios of estimated expression levels for fixed compared to non-fixed cells (column 7).

                                                                   Fixation
                                                       Number of    effect
Delivery                            [EGFP]    Number    retinas      (fold
 method    Promoter    Cell type     (μM)     cells    analyzed    decrease)
--------   --------   -----------   -------   ------   ---------   ---------
Genomic    β-actin    Live rod      270± 12    105         8           -
                      and cone

Genomic    β-actin    Fixed rod     120± 19     31         5          2.3
                      and cone

Genomic    β-actin    Live          440± 60     24         4           -
                      bipolar
Genomic    β-actin    Fixed         220± 14      5         2          2.0
                      bipolar
Genomic    LMHCops    Live cone      60±  9     28         3           -

Genomic    LMHCops    Fixed cone     30±  3     25         3          2.1

Somatic    CMV        Fixed cone     40± 10     11         4
(AAV2/5)

Somatic    CMV        Fixed RPE     680±170     12         1
(AAV2/5)              cell

Somatic    CMV        Fixed         150± 90      3         1
(AAV2/5)              Müller cell

Somatic    CMV        Fixed          65± 10     11         1
(AAV2/5)              amacrine

Somatic    CMV        Fixed          45±  5     12         1
(AAV2/5)              ganglion

Somatic    CMV        Fixed cone     15±  3      2
(AAV2/2)

Somatic    CMV        Fixed RPE     300±110      6
(AAV2/2)              cell

Somatic    CMV        Fixed          20          1
(AAV2/2)              Müller cell

Somatic    CMV        Fixed          45±  5      8
(AAV2/2)              amacrine

Somatic    CMV        Fixed          62± 10      7
(AAV2/2)              ganglion

Rex, Mol Vis 2005; 11:1236-1245 <http://www.molvis.org/molvis/v11/a141/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535