Table 1 of
Rex, Mol Vis 2005;
11:1236-1245.
Table 1. Concentrations of enhanced green fluorescent protein in retinal cells
Column 1 specifies the means by which the enhanced green fluorescent protein cDNA was delivered. Genomic delivery was accomplished through microinjection of zygote pronuclei; column 2 lists the promoter; column 3 gives the cell type. Column 4 gives the estimated EGFP concentration (Figure 2); the values are mean±SEM, with the average taken over the number of cells indicated in column 5. Column 6 shows the numbers of retinas analyzed. The final column provides ratios of estimated expression levels for fixed compared to non-fixed cells (column 7).
Fixation Number of effect Delivery [EGFP] Number retinas (fold method Promoter Cell type (μM) cells analyzed decrease) -------- -------- ----------- ------- ------ --------- --------- Genomic β-actin Live rod 270± 12 105 8 - and cone Genomic β-actin Fixed rod 120± 19 31 5 2.3 and cone Genomic β-actin Live 440± 60 24 4 - bipolar Genomic β-actin Fixed 220± 14 5 2 2.0 bipolar Genomic LMHCops Live cone 60± 9 28 3 - Genomic LMHCops Fixed cone 30± 3 25 3 2.1 Somatic CMV Fixed cone 40± 10 11 4 (AAV2/5) Somatic CMV Fixed RPE 680±170 12 1 (AAV2/5) cell Somatic CMV Fixed 150± 90 3 1 (AAV2/5) Müller cell Somatic CMV Fixed 65± 10 11 1 (AAV2/5) amacrine Somatic CMV Fixed 45± 5 12 1 (AAV2/5) ganglion Somatic CMV Fixed cone 15± 3 2 (AAV2/2) Somatic CMV Fixed RPE 300±110 6 (AAV2/2) cell Somatic CMV Fixed 20 1 (AAV2/2) Müller cell Somatic CMV Fixed 45± 5 8 (AAV2/2) amacrine Somatic CMV Fixed 62± 10 7 (AAV2/2) ganglion |