Table 1 of
Rex, Mol Vis 2005;
11:1236-1245.Table 1 of
Rex, Mol Vis 2005;
11:1236-1245.
Table 1. Concentrations of enhanced green fluorescent protein in retinal cells
Column 1 specifies the means by which the enhanced green fluorescent protein cDNA was delivered. Genomic delivery was accomplished through microinjection of zygote pronuclei; column 2 lists the promoter; column 3 gives the cell type. Column 4 gives the estimated EGFP concentration (Figure 2); the values are mean±SEM, with the average taken over the number of cells indicated in column 5. Column 6 shows the numbers of retinas analyzed. The final column provides ratios of estimated expression levels for fixed compared to non-fixed cells (column 7).
Fixation
Number of effect
Delivery [EGFP] Number retinas (fold
method Promoter Cell type (μM) cells analyzed decrease)
-------- -------- ----------- ------- ------ --------- ---------
Genomic β-actin Live rod 270± 12 105 8 -
and cone
Genomic β-actin Fixed rod 120± 19 31 5 2.3
and cone
Genomic β-actin Live 440± 60 24 4 -
bipolar
Genomic β-actin Fixed 220± 14 5 2 2.0
bipolar
Genomic LMHCops Live cone 60± 9 28 3 -
Genomic LMHCops Fixed cone 30± 3 25 3 2.1
Somatic CMV Fixed cone 40± 10 11 4
(AAV2/5)
Somatic CMV Fixed RPE 680±170 12 1
(AAV2/5) cell
Somatic CMV Fixed 150± 90 3 1
(AAV2/5) Müller cell
Somatic CMV Fixed 65± 10 11 1
(AAV2/5) amacrine
Somatic CMV Fixed 45± 5 12 1
(AAV2/5) ganglion
Somatic CMV Fixed cone 15± 3 2
(AAV2/2)
Somatic CMV Fixed RPE 300±110 6
(AAV2/2) cell
Somatic CMV Fixed 20 1
(AAV2/2) Müller cell
Somatic CMV Fixed 45± 5 8
(AAV2/2) amacrine
Somatic CMV Fixed 62± 10 7
(AAV2/2) ganglion
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