Figure 1 of
Robinson, Mol Vis 2005;
11:1211-1219.
Figure 1. Mass spectrum of the 30 day deamidation sample of GlyIleAsnAlaGly
The peptide was incubated in 37.00 °C, pH 7.4, 0.15 M Tris-HCl buffer for 54 days. It was diluted 100 fold in H2O and then further diluted 3 fold with 49/49/2 H2O/methanol/acetic acid before being introducted by electrospray into the Fourier transform mass spectrometer. Peak A is the first isotopic peak. Peak B is the deamidation product from deamidation of the first isotopic peak. Peak A+1 of the doublet is the second isotopic peak.