Figure 7 of Nguyen, Mol Vis 2005; 11:1183-1199.


Figure 7. Co-localization of Scrib and adhesion proteins in the eye

To determine if Scrib co-localized with E- or N-cadherin throughout the eye, we conducted double immunofluorescence experiments on eye sections from P10 mice. Scrib overlapped with E-cadherin in the corneal epithelium and in the ciliary body. Scrib overlapped with ZO-1 in blood vessels and in the corneal endothelium. The figure shows immunofluorscent staining in the cornea (c; A-H), iris (i) and ciliary body (cb; A,D,E,G) and retina (r; C,F,I) for Scrib and E-cadherin (A-C), Scrib and N-cadherin (D-F), and Scrib and ZO-1 (G-I). Ciliary body and iris (i; A,D,G), cornea (c; B,E,H), and retina (r; C,F,I). Staining for Scrib is red, staining for E-cadherin, N-cadherin, and ZO-1 is green and overlap in staining for Scrib and E-cadherin, N-cadherin or ZO-1 is yellow. B: The corneal epithelium (cep) arrow shows overlap in staining of Scrib and E-cadherin in the corneal epithelium and the arrowhead shows the staining for Scrib in the corneal endothelium. The arrows in C,I show staining in blood vessels in the ganglion cell layer. The arrowhead in G shows overlap in staining in the blood vessels of the iris. The arrowheads in H show overlap in staining in corneal endothelium. The insets in A,B,E,G,H show the detail of overlapping staining patterns in the regions corresponding to the areas in white boxes in the respective panels. The inset in A, arrow, shows the overlap in staining for Scrib and E-cadherin in the outer epithelium of the ciliary body (cboe). E: Arrowhead in inset shows the staining for Scrib in the corneal endothelium. The insets in G,H show the punctate pattern of the overlap in staining for Scrib and ZO-1 in the blood vessels in the iris and in the corneal endothelium, respectively. The cornea (c), ciliary body inner epithelium (cbie), corneal stroma (cs), corneal endothelium (cen), ganglion cell layer (gcl), inner plexiform layer (ipl), inner nuclear layer (inl), outer plexiform layer (opl), outer nuclear layer (onl), external limiting membrane (elm), outer and inner segments of photoreceptors (ps), and retinal pigment epithelium (rpe) are identified. In all panels, the anterior of the eye is oriented at the top. The scale bars represent 50 μm and 25 μm in the insets in A,G,H. The insets in A,B,E,G,H show the detail of overlapping staining patterns in the regions corresponding to the areas in the white boxes in the respective panels. In A, the arrows show overlap in staining for Scrib and E-cadherin in the ciliary body. In B, the arrows and arrowheads overlap in staining for Scrib and E-cadherin in the corneal epithelium and endothelium. In C, the arrow shows overlap in staining for Scrib and E-cadherin in the blood vessels in the retinal ganglion cell layer. In E, the arrowheads show the overlap in staining for Scrib and N-cadherin in the corneal endothelium. In G, the arrowheads show the overlap in staining for Scrib and ZO-1 in the blood vessels in the iris. In H, the arrowheads show overlap in staining for Scrib and ZO-1 in the corneal epithelium and endothelium.

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Nguyen, Mol Vis 2005; 11:1183-1199 <http://www.molvis.org/molvis/v11/a136/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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