Figure 6 of Nguyen, Mol Vis 2005; 11:1183-1199.


Figure 6. Co-localization of Scrib and ZO-1 in the lens

To determine if Scrib co-localized with ZO-1 in the epithelial and fiber cells of the lens, we conducted double immunofluorescence experiments on eye sections from P10 mice. Scrib overlapped with ZO-1 in a punctate pattern on the apical surfaces of the epithelial cells and on the short sides of the fiber cells. The figure shows immunofluorescent staining in the lens for Scrib only (A), ZO-1 only (B), and Scrib and ZO-1 (C-K). A-G are longitudinal sections and H-K are transverse sections showing the regions corresponding to those in D-G. A-C are at low magnification, D-G are at intermediate magnifications, and H-K are at high magnification. Staining for Scrib is red, staining for E-cadherin, N-cadherin and ZO-1 is green and overlap in staining for Scrib and E-cadherin, N-cadherin or ZO-1 is yellow. The arrowheads in C,E show the overlap in staining of Scrib and ZO-1 at the apical interface between the epithelium and fiber cells. The arrowheads in D show the punctate pattern of the overlap in staining for Scrib and ZO-1. The inset in D shows the detail of overlapping staining patterns in the regions corresponding to the areas in white box in D and shows the punctate overlap in Scrib and ZO-1 staining on the apical surface of the epithelial cells. The arrowheads in F,G show overlap in staining for Scrib and ZO-1 in blood vessels outside lens capsule. The arrow in G shows the punctate pattern of the overlap in staining at the posterior in the fiber cells. The arrowheads in H,J,K show the punctate overlapping pattern (yellow) of Scrib and ZO-1 on the short sides of the fibers and in J,K the overlap at the basal attachments with the capsule. In H,I, the arrowheads also show the overlap at the epithelial-fiber interface. The anterior (apical) region of the lens fibers (a), ciliary body (cb), iris (i), lens epithelium (e), lens fiber cells (f), transition zone (tz), midposterior fibers (pf), posterior (basal) of the lens (p), and retina (r) are identified. In all panels, the anterior of the eye is oriented at the top. The scale bars represent 100 μm in A-C; 50 μm in D-G; 25 μm in the inset in D; and 25 μm in H-K.

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Nguyen, Mol Vis 2005; 11:1183-1199 <http://www.molvis.org/molvis/v11/a136/>
©2005 Molecular Vision <http://www.molvis.org/molvis/>
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