Figure 9. Immunohistochemical analysis of mAb 8B11 reactivity in rod- and cone-dominant retinas

Retina/choroid/RPE cryosections of paraformaldehyde-fixed eyes from bovine, human, rat (rod-dominant), and from Xenopus laevis, chicken, Nrl knockout mouse (cone-dominant) were incubated with mAb 8B11 or mouse non-immune IgG, and immunoreactivity was visualized with Alexa Fluor 555-conjugated anti-mouse IgG using fluorescence imaging. Retina/choroid/RPE cryosections from paraformaldehyde eyes were incubated with mAb 8B11 or mouse non-immune IgG, and reactivity was visualized with Alexa Fluor 555-conjugated anti-mouse IgG using fluorescence imaging. Phase contrast (A,D,G,J,M,P), mAb 8B11 reactivity (B,E,H,K,N,Q), non-immune mouse IgG reactivity (C,F,I,L,O,R). Bovine sections are from an amelanotic region of the RPE. The scale bars represent 50 μm.