Figure 3. Schematic of RPE65 amino acid sequence

A: Protein fragments (Regions 1-5) encoded by RPE65 partial cDNAs cloned into pHybLex/Zeo constructs used for expression of fusion proteins in yeast. B: RPE65 antigenic index calculated using the Jameson-Wolf prediction in the DNASTAR program Protean. Positions of synthetic peptides 1-5 corresponding to the five sequences of highest predicted antigenicity in Region 2 (F108 to K236) are shown underlined in the enlarged section. C: Amino acid sequences of the synthetic peptides used in this study.