Figure 2. Immunohistochemical analysis of mAb 8B11 and mAb 1F9 reactivity in wild type and Rpe65 knockout mice

Cryosections were incubated with mAb 8B11 (2 μg/ml) or mAb 1F9 (30 μg/ml) using M.O.M. Peroxidase reagents, with visualization using TSA-Alexa fluor 568 reagents and using fluorescence imaging (1/30 s). Phase contrast images (A,D,G,J); mAb reactivity (B,E,H,K); non-immune mIgG reactivity (C,F,I,L).